Cours de biochimie structurale: Lipides et dérivés isopréniques, Volume 4. Front Cover. Pierre Louisot. Simep éditions, – 93 pages. Cours de biochimie. Biochimie Métabolique: Cours de Cycle de Krebs Cours de la physiologie cardiaque et lymphatique svi s5 Cours de Biochimie structurale svi s3. بسم الله . Spécialité: Biochimie structurale. Présenté par. Guillaume sur l’étude de la paraoxonase humaine hPON1 au cours du service militaire du. Pr. Eric Chabrière.

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The detailed programme is in attachments. This contrasts a number of earlier reports where inhibition of apical pathway led to measurable missorting to the basolateral pathway Scheiffele et al.

This was based on several findings.

Email or Phone Password Forgot account? Interestingly, our method showed that MRS contained considerable amounts of a protein of about 50 kDa, biodhimie was identified as porcine serpin, a leukocyte elastase inhibitor band S1, Fig.

Comparative proteome analysis of secretory proteins from pathogenic and nonpathogenic Listeria species. B Western blotting of galectin-4 in carrier vesicles. The absence of a method for the systematic identification of proteins secreted by LAB, grown in complex media, prompted us to undertake this study. No binding to gangliosides was observed unpublished data. Lactococcus lactis n35 was isolated from a sheep artisanal cheese.

Le Bivic, and E. Results are expressed as molar ratio or percentage of molar ratio. In contrast, basolateral membrane markers were not affected. Distinct transport vesicles mediate the delivery of plasma membrane proteins to the apical and basolateral domains of MDCK cells.


Bibliographic analysis 6 ECTS. Included in this group are some surface-associated proteins that are released into the external medium due to the physiological turnover of the cell wall Turner et al. Carbohydrate-mediated Golgi to cell surface transport and apical targeting of membrane proteins.


Results showed that these ligands corresponded to sulfatides and galactosylceramides syructurale a similar composition as those identified after co-immunoprecipitation from HT 5M12 cells with the anti—galectin-4 antibody.

By confocal microscopy we could demonstrate that galectin-4 was normally localized throughout the cytosol and accumulated in the subapical region of the HT cells.

Glycolipids were isolated from human sciatic nerve Zanetta et al. S2, available at http: Applications must be sent. If possible, students should have also participated in an internship of at least two months in a research laboratory.

Galectin-4 and sulfatides in apical membrane trafficking in enterocyte-like cells

Ap, apical; Bl, basolateral. This article is distributed under the terms of an Attribution—Noncommercial—Share Alike—No Mirror Sites license for the first six months after the publication date see http: Soluble lactose-binding lectin from rat intestine with two different carbohyrate-binding domains in the same peptide chain.

The O -glycosylated stalk domain is required for apical sorting of neurotrophin receptors in polarized MDCK cells. Epithelial cells contain two distinct surfaces with different composition and function. Les cours de M2 sont en anglais, ainsi que les rapports et soutenances de stages. Functional rafts in cell membranes. Searches in databases were done by using the Profound software. In contrast, some LAB strains are not able to grow or grow deficiently in defined media, making difficult the identification of the secreted proteins following those methods.


Ten of them were successfully identified Table 1 etructurale, and nine not identified labeled with asterisks in Biochiimie. Notes Abbreviations used in this paper: Galectin-4 is mostly expressed in the gastrointestinal tract of mammals.

Fine specificity of the domain-I of recombinant tandem-repeat-type galectin-4 from rat gastrointestinal tract G4-N. TGN-derived strcturale were isolated according to the procedure described by Wandinger-Ness et al. Pulse-chase and transport assays Cells were grown on Transwells Costar Data Packaging until day 10 and processed for radiolabeling and cell surface biotinylation Le Bivic et al. December 5 at 8: Heat shock cognate kD protein hsc71 hsp Don’t hesitate to share S1, available at http: The absence of ER contamination in the vesicle preparation was checked by Structufale blotting with an antibody against calnexin Fig.

Previously, we have demonstrated that proteins assumed to be part of the apical delivery mechanism, syntaxin 3 and annexin XIIIb, also accumulated intracellularly after inhibitor treatment Delacour et al.

Western blot analysis The samples were processed as previously described Gouyer et al. Initiation to laboratory research16 weeks 21 ECTS.